DNA Microarrays: A Practical Approach

Capa
Oxford University Press, 1999 - 210 páginas
3 Resenhas
DNA Microarrays: A Practical Approach is the first comprehensive overview of an exciting and powerful new technology. DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA, providing a massive parallel platform for data gathering and representing a fundamental technical advance in biomedical research. Such biochips gather data at an unprecedented rate by enabling the use of advanced fabrication, detection, and data mining technologies. Written and edited by experts in the field, this book provides fascinating insight into this remarkable advancement. It opens with an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips, and continues with descriptions of the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. The chapters address many topics, among them assay optimization, antisense scanning arrays, the manufacture of molecular arrays, and gene expression analysis. Also addressed are the uses of expression data in bioinformatics, of active microelectronic arrays for DNA hybridization analysis, and of microarray technology in pharmacogenomics. DNA Microarrays: A Practical Approach is ideal for researchers investigating patterns of gene expression (and the relationship with disease), and it is essential for any researcher interested in the use of biochips.
 

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Conteúdo

Microarray assays
6
Applications of microarrays
12
Sample handling
25
minimizing the background
29
Instrument performance measures
36
References
42
Generation of libraries from RDA subtractions
51
Use of oligonucleotide arrays in enzymatic
61
Conclusion
118
Generation of aRNAfirst round
126
Acknowledgements
136
The raw data pipeline
146
Medial lexicon and donor sample registration
155
Active microelectronic arrays for
167
Point mutation analysis on active electronic arrays
173
Example of single base discrimination analysis
181

Preparation of target DNA
67
Variations on protocols
73
Application of inkjet printing technology
101
Molecular array fabrication by deposition
111
applications
187
Appendix
203
Direitos autorais

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Referências a este livro

Components of Variance
D.R. Cox,P.J. Solomon
Visualização parcial - 2002
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Sobre o autor (1999)

Mark Schena is at Stanford University.

Informações bibliográficas