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Termos e frases comunsagarose aliquots animals antibodies astrocytes axons BDNF brain cell bodies cell culture cell lines cell suspension cell types centrifuge cerebellar coated cocultures collagen concentration containing culture dish culture medium D-MEM dendrites density differentiation dissection dissociated embryos explants expression fibroblasts figure flask fluorescent forceps ganglia ganglion gene Gibco glass coverslips glial cells granule cells growth cones growth factors hippocampal hippocampal neurons incubator injection labeled laminin membrane methods microisland microscope myelination nerve neural neurites neuronal cultures neurons neurotrophic neurotrophins nonneuronal cells O-2A progenitor cells obtained oligodendrocytes optic outgrowth Pasteur pipette PC12 cells Petri dish phenotype pipette plastic plating polylysine prepared procedure protein protocol purified Purkinje cells receptors removed Rinse Scale bar Schwann cells serum serum-free Sigma slice cultures solution spinal staining sterile studies substrate surface survival sympathetic neurons synaptic technique tissue tissue-culture toxic transfection trituration trypsin tube vectors viral virus vitro Passagens mais conhecidasPágina 616 - Greene, LA, and Tischler, AS (1976). Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Página 616 - A new technique for the assay of infectivity of human adenovirus 5 DNA. Página 605 - Chomczynski, P. and Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Página 621 - Jessell TM, Siegel RE, and Fischbach GD (1979) Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. Proc. Natl. Acad. Sci. Página 640 - A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303... Página 618 - Hatten, ME (1985) Neuronal regulation of astroglial morphology and proliferation in vitro. J. Cell Biol. Página 614 - TC 1994. Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse. Página 612 - Friedrich, G., and Soriano, P. (1991). Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes Dev. 5, 1513-1523. Página 630 - Primary cultures of dissociated sympathetic neurons. I. Establishment of long-term growth in culture and studies of differentiated properties, J. Página 620 - Honig, MG, and Hume, RI (1986). Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J. Cell. Biol. 103, 171-187. Referências a este livroDe outros livros
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