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Culturing nerve cells

Capa
Gary Banker, Kimerly Goslin
0 Resenhas
MIT Press, 1998 - 666 páginas
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.

The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.

Contributors:
Janet Alder, Hannelore Asmussen, Gerard Bain, Gary Banker, Robert W. Baughman, Richard P. Bunge, Ann Marie Craig, Matthew E. Cunningham, Dominique Debanne, Stephen E. Farinelli, Michael F. A. Finley, Gerald D. Fishbach, Beat H. Gahwiler, W.-Q. Gao, Daniel J. Goldberg, Kimberly Goslin, David I. Gottlieb, Lloyd A. Greene, Mary Beth Hatten, Dennis Higgins, James E. Huettner, Kenneth A. Jones, Naomi Kleitman, Raul Krauss, Ronald M. Lindsay, Nagesh K. Mahanthappa, Carol A. Mason, Margot Mayer-Proschel, R. Anne McKinney, Mary E. Morrison, Mark Noble, David S. Park, Paul H. Patterson, Mu-ming Poo, Richard T. Robertson, Samuel Schacher, Michael M. Segal, Carolyn L. Smith, Nacira Tabti, Scott M. Thompson, Roseann Ventimiglia, Ginger S. Withers, Patrick M. Wood, Min Yao.
  

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Conteúdo

Getting Started
3
Types of Nerve Cell Cultures Their Advantages and Limitations
11
Primary Dissociated Cell Cultures
37
Transfecting Cultured Neurons
79
Characterizing and Studying Neuronal Cultures
113
Choosing the Right System
157
Neuronlike Cells Derived in Culture from PI 9 Embryonal Carcinoma
189
Culturing the Large Neurons of Aplysia
213
Mass Cultures and Microislands of Neurons from Postnatal Rat Brain
309
Rat Hippocampal Neurons in LowDensity Culture
339
Rat Striatal Neurons in LowDensity SerumFree Culture
371
Cell Culture of Cholinergic and Cholinoceptive Neurons from the Medial
395
Purification and Coculture of Identified Cell Populations
419
Organotypic Slice Cultures of Neural Tissue 441
461
Culture of Astrocytes Oligodendrocytes and thusProgenitor Cells
499
Tissue Culture Methods for the Study of Myelination
559

Culturing Spinal Neurons and Muscle Cells from Xenopus Embryos
237
Cultures from Chick Peripheral Ganglia Ml
261
Culturing Mammalian Sympathoadrenal Derivatives
289
References
595
Index
659
Direitos autorais

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Termos e frases comuns

Passagens mais conhecidas

Página 616 - Greene, LA, and Tischler, AS (1976). Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Página 616 - A new technique for the assay of infectivity of human adenovirus 5 DNA.
Página 605 - Chomczynski, P. and Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Página 621 - Jessell TM, Siegel RE, and Fischbach GD (1979) Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. Proc. Natl. Acad. Sci.
Página 640 - A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303...
Página 618 - Hatten, ME (1985) Neuronal regulation of astroglial morphology and proliferation in vitro. J. Cell Biol.
Página 614 - TC 1994. Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse.
Página 612 - Friedrich, G., and Soriano, P. (1991). Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes Dev. 5, 1513-1523.
Página 630 - Primary cultures of dissociated sympathetic neurons. I. Establishment of long-term growth in culture and studies of differentiated properties, J.
Página 620 - Honig, MG, and Hume, RI (1986). Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J. Cell. Biol. 103, 171-187.

Referências a este livro

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Referências de páginas web

Culturing Nerve Cells, Second Edition - The MIT Press
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
mitpress.mit.edu/ catalog/ item/ default.asp?tid=8375& ttype=2

JSTOR: Culturing Nerve Cells.
CULTURING NERVE CELLS. Cellular and Molecular Neuroscience Series. A Bradford Book. Edited by Gary Banker and Kimberly, Goslin; Series Editor: Charles F. ...
links.jstor.org/ sici?sici=0033-5770(199303)68%3A1%3C141%3ACNC%3E2.0.CO%3B2-J

Springer Protocols: Abstract: Culturing Primary and Transformed ...
(1998) Culturing Nerve Cells, 2nd ed. MIT Press, Cambridge, MA. 2. Fedoroff, S. and Richardson, A. (eds.) (2001) Protocols for Neural Cell Culture, 3rd ed. ...
www.springerprotocols.com/ Abstract/ doi/ 10.1385/ 1-59259-848-X:299

IMAGING AND MANIPULATING LIVING NEURONS WITH ATOMIC FORCE MICROSCOPY
Cultures“, in Culturing Nerve Cells, G. Banker and. K. Goslin, Eds. Cambridge. MA: MIT Press, 1998),. pp. 71. [12] D. Higgins and G. Banker, ...
ieeexplore.ieee.org/ iel5/ 9639/ 30463/ 01404243.pdf

Coverslip holder - US Patent 5635396
A reference text entitled, Culturing Nerve Cells, G. Banker and K. Goslin, ... In the "protocol" from the reference Culturing Nerve Cells, the authors ...
www.patentstorm.us/ patents/ 5635396-description.html

DRG cell culture - Neuroscience
Referred to "Culturing Nerve Cells" Banker and Goslin, Editors. In fact the key point is how to find the DRG. -noirvan- ...
www.protocol-online.org/ biology-forums/ posts/ 10411.html

Atomic Force Microscopy Applications to Neuroscience Helen A ...
[9] nk Mahanthappa and ph Patterson, in Culturing Nerve Cells, edited by G. Banker and K. Goslin (The MIT Press, Cambridge, MA, 1998), pp. 289. ...
www.mrs.org/ s_mrs/ bin.asp?CID=2672& DID=67640& DOC=FILE.PDF

Hippocampal Cell Culture
Adapted from Culturing Nerve Cells, 2nd Edition; Banker and Goslin 1998. pages 352-356. Note, we have switched from the glia feeder layer to neurobasal ...
bio.research.ucsc.edu/ people/ hinck/ hippoprep.html

Journal of Neuroscience Methods : A novel and rapid method for ...
In: Banker G, Goslin K, editors, Culturing nerve cells, mit Press, Cambridge, 1991, pp. 309–35. Lim R, Miller JF. Isolation of rat astroblasts. ...
linkinghub.elsevier.com/ retrieve/ pii/ S0165027097001982

Fast, convenient, and effective method to transiently transfect ...
Culturing nerve cells,. 2nd ed. Cambridge, MA: MIT Press. p 339–370. Graham FL, van der Eb AJ. 1973. A new technique for the assay of ...
doi.wiley.com/ 10.1002/ (SICI)1097-4547(19991215)58:6%3C831::AID-JNR10%3E3.3.CO;2-D

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