Developmental Biology Protocols: Volume III

Capa
Rocky S. Tuan, Cecilia W. Lo
Springer Science & Business Media, 2 de fev. de 2008 - 522 páginas
Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
 

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Conteúdo

INTRODUCTION
5
DEVELOPMENTAL PATTERN AND MORPHOGENESIS
10
Key Molecules
11
Apoptosis in Development
13
Jenny E Rooke Nicole A Theodosiou and Tian Xu
15
EMBRYO STRUCTURE AND FUNCTION
16
Isolation of Neuroepithelium and Formation of Minispheres
19
Optical Coherence Tomography Imaging in Developmental Biology
22
Vahe Bedian
159
PART IVM ODELS OF MORPHOGENESIS AND DEVELOPMENT
168
Amphibian Organizer Activity
178
Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration
198
Eric Petitclerc Tami von Schalscha and Peter C Brooks 23 Analysis of Embryonic Vascular Morphogenesis
213
EpithelialMesenchymal Interactions
229
Methods for Manipulating the Chick Limb Bud to Study Gene Expression
238
In Vitro Procedures
268

Application of Antisense Oligodeoxynucleotides in Developing
23
I Normal Development
25
Acquisition Display and Analysis of Digital 3D and TimeLapse
26
Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel
29
Cell Lineage Analysis in Xenopus Embryos
32
Transgenic Manipulation of the Sea Urchin Embryo
35
NCadherin
37
Mouse Chimeras and the Analysis of Development
38
Interspecific MouseChick Chimeras
41
Gene Expression Analyzed by Ribonuclease Protection Assay
45
XX
46
Retroviral Gene Transduction in Limb Bud Micromass Cultures
48
Relative Reverse TranscriptionPolymerase Chain Reaction
51
Gene Expression Analysis Using Quantitative Reverse Transcription
59
Radioactive Probes
87
Practical Aspects and Quantification
97
Index
109
mRNA and Protein CoLocalization on Tissue Sections by Sequential
117
WholeMount In Situ Hybridization to Study Gene Expression During
125
Multicolor WholeMount In Situ Hybridization
139
Combined In Situ
149
In Vitro Fertilization
277
An In Vitro Model for Trophoblast Giant
301
Bone MarrowDerived Mesenchymal Progenitor Cells
313
Preparation of Chick Striated Muscle Cultures
337
Study of Skeletal Myogenesis in Cultures of Unsegmented
351
Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model
359
Mesenchymal Cells
377
Murine C3H10T12 Multipotential Cells as an In Vitro Model
383
Adesola Majolagbe and Pamela Gehron Robey
391
Studying Early Hematopoiesis Using Avian Blastoderm Cultures
399
CadherinMediated CellCell Interactions
409
Analysis of Hyaluronan Using Biotinylated HyaluronanBinding Proteins
441
Microinjection of Fluorescently Labeled αActinin into Living Cells
449
Pax3 and Vertebrate Development
459
SCREENING AND MAPPING OF NOVEL GENES AND MUTATIONS
468
Collagen Type X
471
Targeting Mutations
491
Transgenic Mouse Models of Craniofacial Disorders
499
Index
513
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