Developmental Biology Protocols: Volume IIIRocky S. Tuan, Cecilia W. Lo Springer Science & Business Media, 2 de fev. de 2008 - 522 páginas Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium. |
Conteúdo
5 | |
DEVELOPMENTAL PATTERN AND MORPHOGENESIS | 10 |
Key Molecules | 11 |
Apoptosis in Development | 13 |
Jenny E Rooke Nicole A Theodosiou and Tian Xu | 15 |
EMBRYO STRUCTURE AND FUNCTION | 16 |
Isolation of Neuroepithelium and Formation of Minispheres | 19 |
Optical Coherence Tomography Imaging in Developmental Biology | 22 |
Vahe Bedian | 159 |
PART IVM ODELS OF MORPHOGENESIS AND DEVELOPMENT | 168 |
Amphibian Organizer Activity | 178 |
Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration | 198 |
Eric Petitclerc Tami von Schalscha and Peter C Brooks 23 Analysis of Embryonic Vascular Morphogenesis | 213 |
EpithelialMesenchymal Interactions | 229 |
Methods for Manipulating the Chick Limb Bud to Study Gene Expression | 238 |
In Vitro Procedures | 268 |
Application of Antisense Oligodeoxynucleotides in Developing | 23 |
I Normal Development | 25 |
Acquisition Display and Analysis of Digital 3D and TimeLapse | 26 |
Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel | 29 |
Cell Lineage Analysis in Xenopus Embryos | 32 |
Transgenic Manipulation of the Sea Urchin Embryo | 35 |
NCadherin | 37 |
Mouse Chimeras and the Analysis of Development | 38 |
Interspecific MouseChick Chimeras | 41 |
Gene Expression Analyzed by Ribonuclease Protection Assay | 45 |
XX | 46 |
Retroviral Gene Transduction in Limb Bud Micromass Cultures | 48 |
Relative Reverse TranscriptionPolymerase Chain Reaction | 51 |
Gene Expression Analysis Using Quantitative Reverse Transcription | 59 |
Radioactive Probes | 87 |
Practical Aspects and Quantification | 97 |
Index | 109 |
mRNA and Protein CoLocalization on Tissue Sections by Sequential | 117 |
WholeMount In Situ Hybridization to Study Gene Expression During | 125 |
Multicolor WholeMount In Situ Hybridization | 139 |
Combined In Situ | 149 |
In Vitro Fertilization | 277 |
An In Vitro Model for Trophoblast Giant | 301 |
Bone MarrowDerived Mesenchymal Progenitor Cells | 313 |
Preparation of Chick Striated Muscle Cultures | 337 |
Study of Skeletal Myogenesis in Cultures of Unsegmented | 351 |
Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model | 359 |
Mesenchymal Cells | 377 |
Murine C3H10T12 Multipotential Cells as an In Vitro Model | 383 |
Adesola Majolagbe and Pamela Gehron Robey | 391 |
Studying Early Hematopoiesis Using Avian Blastoderm Cultures | 399 |
CadherinMediated CellCell Interactions | 409 |
Analysis of Hyaluronan Using Biotinylated HyaluronanBinding Proteins | 441 |
Microinjection of Fluorescently Labeled αActinin into Living Cells | 449 |
Pax3 and Vertebrate Development | 459 |
SCREENING AND MAPPING OF NOVEL GENES AND MUTATIONS | 468 |
Collagen Type X | 471 |
Targeting Mutations | 491 |
Transgenic Mouse Models of Craniofacial Disorders | 499 |
513 | |
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