Developmental Biology Protocols: Volume IIRocky S. Tuan, Cecilia W. Lo Springer Science & Business Media, 5 de fev. de 2008 - 505 páginas Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium. |
Conteúdo
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5 | |
Radioactive Probes | 10 |
Bioassays of Inductive Interactions in Amphibian Development | 11 |
WholeMount In Situ Hybridization to Study Gene Expression During Mouse | 13 |
Bioassays for Studying the Role of the Peptide Growth Factor Activin | 15 |
Confocal Microscopy of Live Xenopus Oocytes Eggs and Embryos | 17 |
Amphibian Oganizer Activity | 19 |
Neural Tube Development | 167 |
Isolation of Neuroepithelium and Formation of Minispheres | 179 |
Examination of Normal and Abnormal Placentation in the Mouse | 185 |
Magnetic Resonance Imaging Analysis of Embryos | 203 |
of Murine Developmental Limb Abnormalities | 219 |
Assessment of Laterality Defects in Rodent Embryos | 227 |
Cardiac Morphogenesis and Dysmorphogenesis | 239 |
Heart | 261 |
Ultrasound Backscatter Microscopy of Mouse Embryos | 23 |
Methods for Manipulating the Chick Lumb Bud to Study Gene | 25 |
Analysis of Mammary Gland Morphogenesis | 27 |
An Invitro Model for Trophoblast Giant | 28 |
Photoactivatable Caged Fluorescein as a Cell Tracer for Fate | 33 |
ElectroporationMediated DNA Transfection of Embryonic Chick Limb | 34 |
Specification of Cardiac Mesenchyme and Heart Morphogenesis | 39 |
Analysis of Hyaluronan Using Biotinylated HyaluronanBinding Proteins | 40 |
Interspecific MouseChick Chimeras | 41 |
Transgenic Mouse Models of Cranofacial Disorders | 45 |
Retroviral Gene Transduction in Limb Bud Micromass Cultures | 48 |
Craniofacial Skeletel Morphogenesis In Vitro | 55 |
Transplantation and Culture Techniques for the Analysis | 71 |
Embryonic Chicken Skin Explant Cultures | 101 |
Apoptosis in Development | 107 |
Methods to Detect Patterns of Cell Death in Drosophila | 115 |
Mechanisms of Neurulation | 125 |
Neurulation and Neural Tube Closure Defects | 135 |
Index | 139 |
Neural Tube Defects | 161 |
Biologically Based Risk Assessment Models | 271 |
Positional Cloning | 285 |
Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmen | 297 |
PCRBased Cloning of Cortically Localized RNAs from Xenopus Oocytes | 309 |
Analysis of mRNA Expression During Preimplantation Development | 315 |
Differential Screens with Subtracted PCRGenerated cDNA Libraries from | 333 |
Thomas B Knudsen | 345 |
Production of Transgenic Drosophila | 353 |
Transgenic Manipulation of the Sea Urchin Embryo | 365 |
Shuo Lin | 375 |
Production of Avian Chimeras and Germline Transmission | 385 |
Qingzia Wei Kristin L Woods and Robert J Etches 40 Nuclear Transplantation and Cloning in Mammals | 399 |
Jean Richa | 427 |
Production of Transgenic Mice with Yeast Artificial Chromosome | 435 |
GeneTargeting Strategies | 455 |
Chimeric Animals and Germline Transmission | 465 |
45 | 477 |
SiteSpecific Recombination | 487 |
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