Developmental Biology Protocols: Volume II

Capa
Rocky S. Tuan, Cecilia W. Lo
Springer Science & Business Media, 5 de fev. de 2008 - 505 páginas
Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
 

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Overview II
4
IN VITRO MODELS AND ANALYSIS DIFFERENTIATION
5
Radioactive Probes
10
Bioassays of Inductive Interactions in Amphibian Development
11
WholeMount In Situ Hybridization to Study Gene Expression During Mouse
13
Bioassays for Studying the Role of the Peptide Growth Factor Activin
15
Confocal Microscopy of Live Xenopus Oocytes Eggs and Embryos
17
Amphibian Oganizer Activity
19
Neural Tube Development
167
Isolation of Neuroepithelium and Formation of Minispheres
179
Examination of Normal and Abnormal Placentation in the Mouse
185
Magnetic Resonance Imaging Analysis of Embryos
203
of Murine Developmental Limb Abnormalities
219
Assessment of Laterality Defects in Rodent Embryos
227
Cardiac Morphogenesis and Dysmorphogenesis
239
Heart
261

Ultrasound Backscatter Microscopy of Mouse Embryos
23
Methods for Manipulating the Chick Lumb Bud to Study Gene
25
Analysis of Mammary Gland Morphogenesis
27
An Invitro Model for Trophoblast Giant
28
Photoactivatable Caged Fluorescein as a Cell Tracer for Fate
33
ElectroporationMediated DNA Transfection of Embryonic Chick Limb
34
Specification of Cardiac Mesenchyme and Heart Morphogenesis
39
Analysis of Hyaluronan Using Biotinylated HyaluronanBinding Proteins
40
Interspecific MouseChick Chimeras
41
Transgenic Mouse Models of Cranofacial Disorders
45
Retroviral Gene Transduction in Limb Bud Micromass Cultures
48
Craniofacial Skeletel Morphogenesis In Vitro
55
Transplantation and Culture Techniques for the Analysis
71
Embryonic Chicken Skin Explant Cultures
101
Apoptosis in Development
107
Methods to Detect Patterns of Cell Death in Drosophila
115
Mechanisms of Neurulation
125
Neurulation and Neural Tube Closure Defects
135
Index
139
Neural Tube Defects
161
Biologically Based Risk Assessment Models
271
Positional Cloning
285
Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmen
297
PCRBased Cloning of Cortically Localized RNAs from Xenopus Oocytes
309
Analysis of mRNA Expression During Preimplantation Development
315
Differential Screens with Subtracted PCRGenerated cDNA Libraries from
333
Thomas B Knudsen
345
Production of Transgenic Drosophila
353
Transgenic Manipulation of the Sea Urchin Embryo
365
Shuo Lin
375
Production of Avian Chimeras and Germline Transmission
385
Qingzia Wei Kristin L Woods and Robert J Etches 40 Nuclear Transplantation and Cloning in Mammals
399
Jean Richa
427
Production of Transgenic Mice with Yeast Artificial Chromosome
435
GeneTargeting Strategies
455
Chimeric Animals and Germline Transmission
465
45
477
SiteSpecific Recombination
487
Index
497
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