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PCR

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Taylor & Francis, 25/01/2007 - 292 páginas
A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
  

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Páginas selecionadas

Conteúdo

1 An introduction to PCR
1
2 Understanding PCR
9
3 Reagents and instrumentation
23
4 Optimization of PCR
65
5 Analysis sequencing and in vitro expression of PCR products
87
6 Purification and cloning of PCR products
111
7 PCR mutagenesis
137
8 Analysis of gene expression
185
9 Realtime RTPCR
209
10 Cloning genes by PCR
233
11 Genome analysis
257
Index
283
Direitos autorais

Outras edições - Visualizar todos

Pcr
Pcr
M. J. McPherson,Simon Geir Moller
Não há visualização disponível - 2006

Termos e frases comuns

adaptor addition agarose gel aliquot alleles allow amino acid amplicon amplification products amplify analysis annealing temperature approach appropriate assay base pairs buffer cells Chapter cloning codon coli complementary concentration contain defined denaturation detection difficult DNA fragments DNA sequence DNA synthesis dNTPs double-stranded efficient ensure example exonuclease fidelity Figure final first first-strand cDNA flanking gel electrophoresis genomic genomic DNA hybridization identified increase incubation insertion labeled ligation microcentrifuge modified molecular beacons mRNA mutagenesis mutations nested PCR nonspecific Northern blot Nucleic Acids nucleotides oligo-dT oligonucleotide oligonucleotide primers optimization PCR amplification PCR cycle PCR primers PCR products PCR reaction performed plasmid polymorphisms primers primers P1 probe proofreading protein Protocol purification reagents real-time RT-PCR recombinant region restriction digestion restriction enzyme reverse transcriptase RT-PCR samples single-stranded specific standard step strand sufficient Taq DNA polymerase TaqMan target gene template DNA thermal cycler thermostable DNA polymerase transcription tube vector vectorette wild-type

Sobre o autor (2007)

Dr. Simon Moller, Department of Biology, University of Leicester, UK

Professor Michael McPherson, School of Biochemistry and Microbiology, University of Leeds, UK

Informações bibliográficas

QR code for PCR
TítuloPCR
THE BASICS (Garland Science)
AutoresMike McPherson, Simon Møller
Edição2, ilustrada, revisada
EditoraTaylor & Francis, 2007
ISBN0203002679, 9780203002674
Num. págs.292 páginas
  
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