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Molecular cloning:

a laboratory manual, Volume 2
Capa
4 Resenhas
Cold Spring Harbor Laboratory, 1989

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Review: Molecular Cloning: A Laboratory Manual

Comentário do usuário  - Adrianna - Goodreads

Important book for someone in molecular biology field Ler resenha completa

Review: Molecular Cloning: A Laboratory Manual

Comentário do usuário  - Sofia - Goodreads

This is the closest thing a molecular biologist has to Holy Scripture. There isn't a single molbio lab in the world that doesn't worship at the altar of Saint Sambrook, patron of DNA, RNA and protein. Goodreads needs a new rating system for this, because five stars isn't enough. Ler resenha completa

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Conteúdo

TEST LIGATION 3 29
8-3
DNA METHYLATION 5
8-5
SYNTHESIS OF THE FIRST STRAND OF cDNA 8
8-11

84 outras seções não mostradas

Termos e frases comuns

Acad acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography Cl pH cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA hybridization Klenow fragment labeled length ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence termini transcription transfer vector

Informações bibliográficas

QR code for Molecular cloning
TítuloMolecular cloning: a laboratory manual, Volume 2
Molecular Cloning: A Laboratory Manual, E. F. Fritsch
AutoresJoseph Sambrook, E. F. Fritsch, Tom Maniatis
Edição2
EditoraCold Spring Harbor Laboratory, 1989
Original deUniversidade de Michigan
Digitalizado23 fev. 2010
  
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